Project P16

Proteomic analysis of brown adipocyte organelle dynamics and contact site formation

© Krahmer

In BAT, mitochondria and LDs form extensive contact sites to orchestrate lipid metabolism. To date, the LD-mitochondrial contact site proteome, its dynamics and regulation remains elusive. We established an organelle proteomics workflow to generate a global map of protein expression, modification and localization upon induction of thermogenesis. We will combine proteomics with cryo-electron tomography and super-resolution microscopy to study the architecture of these contact sites. Combining state-of-the-art techniques, this project will unravel the organelle proteomes and enable studies on dynamic organelle interaction in BAT.

Project Leaders

Avatar Krahmer

Natalie Krahmer


Helmholtz Diabetes Center

Ingolstädter Landstraße 1

85764 Neuherberg

Cellular Proteomics and Metabolic Signaling

Emmy-Noether Research Group

Institute for Diabetes and Obesity

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